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Chromatography

Chromatography

by 317306 Dunna Shivani -
Number of replies: 2

Why mixture of solvents are to be used in Mobile phase ???

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In reply to 317306 Dunna Shivani

Re: Chromatography

by 317319 Seelamsetty Satya Sree -

Non- polar molecules in the mixture that are to be seperated 

Will have little attraction for the water molecules attached to the cellulose and so will spend most of their time dissolved in the moving solvent

In reply to 317306 Dunna Shivani

Re: Chromatography

by 317316 Perumalla Vasundhara -

The mobile phase flows through the stationary phase and carries the components of the mixture with it. ...Mobile Phases in Normal PhaseChromatography Typically non polarsolvents such as hexane, heptane, iso-octane are used in combination with slightly more polar solvents such as isopropanol, ethyl-acetate or chloroform