- Adsorption chromatography is the oldest types of chromatography technique. It makes use of a mobile phase which is either in liquid or gaseous form. The mobile phase is adsorbed onto the surface of a stationary solid phase.
- The degree of a chemical's retention in adsorption chromatography will depend on
- (1) the binding strength of this chemical to the support,
- (2) the surface area of the support,
- (3) the amount of mobile phase displaced from the support by the chemical, and
- (4) the binding strength of the mobile phase to the support.
- Electrostatic interactions, hydrogen bonding, dipole-dipole interactions, and dispersive interactions (ie, van der Waals forces) all may affect retention in this type of chromatography.
(Edited by Sai Kumar S V - original submission Monday, 5 August 2019, 11:12 PM)