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S

Sample


A mixture containing two or more molecular species that needs to be separated for analysis.


SELECTIVITY FACTOR

IT IS DEFINED AS THE RATIO OF PARTITION RATIO OF THE MORE STRONGLY RETAINED SOLUTE TO THE PARTITION RATIO OF LESS STRONGLY HELD SOLUTE 


Sequential elution

sequential elution liquid chromatography, an approach in which two or more elution modes are employed in series for the separation of two or more groups of compounds, can be used to separate not only weak acids (or weak bases) from neutral compounds, but weak acids and weak bases from neutral compounds


Size exclusion

Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. 


Solubility

Amount of a substance (called the solute) that dissolves in a unit volume of a liquid substance (called the solvent) to form a saturated solution under specified conditions of temperature and pressure. Solubility is expressed usually as moles of solute per 100 grams of solvent.


SOLVENT FRONT

In paper chromatography, the wet moving edge of the solvent that progresses along the surface where the separation of the mixture is occurring.

Spectroscopy

Spectroscopy is the study of the interaction between matter and electromagnetic radiation (via electron spectroscopyatomic spectroscopy, etc).

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Stationary phase

The difference in the the rate at which the components of the mixture move through porous medium