Pharmaceutical Analysis

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It is the ratio of total moles of sample in SP to  total moles of sample in MP

Separation of optical isomers can be done by using chiral stationary phases.Different principles operate for different types of stationary phases and for different samples.The stationary phases used for this type of chromatography are mostly chemically bonded silica gel.

Chromatogram is a visible record (such as graph) showing the result of separating the conponents of a mixture by chromatography

Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate.

Chromatography Detector is a device used in liquid Chromatography or gas Chromatography to detect components of the  mixture  being eluted off the Chromatography column There are two general types of detectors destructive and non-destructive.

The functional group containing multiple bonds capable of absorbing radiation .

chromatography in which the substances to be separated are introduced onto the top of a column packed with an adsorbent (as silica gel or alumina), pass through the column at different rates that depend on the affinity of each substance for the adsorbent and for the solvent or solvent mixture, and are usually collected in solution as they pass from the column at different times

Bonded phase : 

Where stationary phase is chemically bonded to a inert support